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KMID : 0613820020120050584
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Journal of Life Science
2002 Volume.12 No. 5 p.584 ~ p.588
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Development of Asymmetric Resolution System for the Production of Chiral Styrene Oxide by Microbial Epoxide Hydrolase
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Lee Ji-Won
Yoon Yeo-Joon
Lee Eun-Yeol
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Abstract
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Asymmetric enantioselective resolution system using epoxide hydrolase activity of Aspergillus niger LK was developed and operated for the production of optically pure styrene oxide. Two-phase hollow-fiber reactor system was employed for the enhanced solubility of racemic styrene oxide in organic phase and protection of epoxide hydrolase activity in aqueous phase. For the removal of phenyl-1,2-ethandiol, the inhibitor of epoxide hydrolase, cascade hollow-fiber reactor system was also developed. Chiral (S)-styrene oxide (39 mM in dodecane) could be asymmetrically resolved with high enantiopurity (> 99% ee) using these reactor system.
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KEYWORD
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asymmetric resolution, hollow-fiber reactor, chiral styrene oxide, Aspergillus niger LK
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